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Catalog Number | orb392435 |
---|---|
Category | Proteins |
Description | Recombinant mouse SEMA4A protein is produced by our mammalian expression system and the target gene encoding Thr33-His682 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SEMA4A |
Entrez | 20351 |
UniProt ID | Q62178 |
Protein Sequence | TGGQGPMPRV KYHAGDGHRA LSFFQQKGLR DFDTLLLSDD GNTLYVGARE AVLALNIQNP GIPRLKNMIP WPASERKKTE CAFKKKSNET QCFNFIRVLV SYNATHLYAC GTFAFSPACT FIELQDSLLL PILIDKVMDG KGQSPFDPVH KHTAVLVDGM LYSGTMNNFL GSEPILMRTL GSQPVLKTDI FLRWLHADAS FVAAIPSTQV VYFFFEETAS EFDFFEELYI SRVAQVCKND VGGEKLLQKK WTTFLKAQLL CAQPGQLPFN IIRHAVLLPA DSPSVSRIYA VFTSQWQVGG TRSSAVCAFS LTDIERVFKG KYKELNKETS RWTTYRGSEV SPRPGSCSMG PSSDKALTFM KDHFLMDEHV VGTPLLVKSG VEYTRLAVES ARGLDGSSHV VMYLGTSTGS LHKAVVPQDS SAYLVEEIQL SPDSEPVRNL QLAPAQGAVF AGFSGGIWRV PRANCSVYES CVDCVLARDP HCAWDPESRL CSLLSGSTKP WKQDMERGNP EWVCTRGPMA RSPRRQSPPQ LIKEVLTVPN SILELPCPHL SALASYHWSH GRAKISEASA TVYNGSLLLL PQDGVGGLYQ CVATENGYSY PVVSYWVDSQ DQPLALDPEL AGVPRERVQV PLTRVGGGAS MAAQRSYWPH VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | SEMAB; SEMB; Semaphorin-4A; Semaphorin-B; Sema B Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
70-90 KDa, reducing conditions |
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