SATB1 Antibody / Special AT-rich sequence-binding protein 1

SKU: orb1823661

Description

The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the SATB1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: PCRP-SATB1-2C3
• Immunogen: Recombinant full-length human SATB1 protein was used as the immunogen for the SATB1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with SATB1 antibody (green, clone PCRP-SATB1-2C3) and phalloidin (red).

Flow cytometry testing of PFA-fixed human HeLa cells with SATB1 antibody (clone PCRP-SATB1-2C3) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free SATB1 antibody (clone PCRP-SATB1-2C3) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q01826