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SARS-CoV-2 (COVID-19) Spike Antibody

SKU: orb1239976

Description

SARS-CoV-2 (COVID-19) Spike Antibody

Research Area

Infectious Disease & Virology

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
ReactivityVirus
Application Notes
WB: 1 μg/mL; IF: 1 μg/mL. IHC: 0.2 μg/mL; Antibody validated: Immunofluorescence and Western blot in human samples. Immunohistochemistry and Immunofluorescence in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL. The immunogen for this is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa). Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins). This antibody can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. All other applications and species not yet tested.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-SARS-CoV-2 (COVID-19) Spike antibody (orb1239976) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
TargetS
PurificationSARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration1 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

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Quality Guarantee

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQHD43416

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.02 mg
$ 240.00
0.1 mg
$ 680.00
Free Secondary Antibody (20 ul)0/0

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