S100A12 antibody

• Catalog Number: orb2276340
• Category: Antibodies
• Description: S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: P80511
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative. Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels) Cellular Localization: Intracellular, Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-812M
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
• Note: For research use only

Colon Colorectal adenocarcinoma showing numerous S100A12 positive granulocytes in the stroma and within tumor glands.

Spleen S100A12 immunostaining is seen in granulocytes of mainly the red pulp.

Stomach Gastric adenocarcinoma densly infiltrated by S100A12 positive granulocytes.