S100-A8 / MRP8 Polyclonal Antibody

• Catalog Number: orb1417584
• Category: Antibodies
• Description: Rabbit polyclonal antibody to S100-A8.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Rat
• UniProt ID: P27005
• Tested applications: ELISA, IF
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein S100-A8, Calgranulin-A, Chemotactic cytoki
• Research Area: Apoptotic, Autophagic, Cancer, Epigenetics, Immuno
• Note: For research use only

Blank control: K562.Primary Antibody (green line): Rabbit Anti-S100-A8 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.

Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-S100-A8 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.