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RUNX1 Rabbit Polyclonal Antibody
SKU: orb592889
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human RUNX1 |
| Target | RUNX1 |
| Molecular Weight | 49 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, CBF2alpha, AML1-EVI-1, PEBP2alpha
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Quality Guarantee
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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.
Sample Tissue: Human MCF7 Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Type: Mouse embryonic d13 spinal motor neurons, Primary Antibody Dilution: 1:1000, Secondary Antibody: Donkey anti rabbit IgG Alexa 594, Secondary Antibody Dilution: 1:1000, Color/Signal Descriptions: RUNX1: Red LSL1: Green, Gene Name: RUNX1.
WB Suggested Anti-RUNX1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: SH-SYSY cell lysate.
WB Suggested Anti-RUNX1 Antibody Titration: 5% Milk, ELISA Titer: dilution: 1:500, Positive Control: Human LCL and mouse brains.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
RUNX1 Rabbit Polyclonal Antibody (orb592889)
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