Rreb1 Antibody

SKU: orb344631

Description

RREB1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:15,000, WB: 1:500 - 1:2,000
• Reactivity: Mouse
• Application Notes:
  This Protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 90-180 kDa in size corresponding to RREB1 by western blotting in the appropriate cell lysate or extract. Multiple bands seen in the above western blot may indicate cross-reactive isoforms or truncated protein products.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-RREB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to mouse RREB1 protein.
• Target: Rreb1
• Purity: Anti-RREB1 antibody is directed against the mouse RREB1 protein. The product was Protein A purified from monospecific antiserum by immunoaffinity purification. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-RREB1 Antibody, rabbit anti-RREB 1 Antibody, FINB antibody, Finger protein in nuclear bodies antibody, LZ321 antibody, Raf responsive zinc finger protein LZ321 antibody, RAS responsive element binding protein 1 antibody

Western blot using Biorbyt's Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 µg per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX.

Quick Database Links

Gene Symbol

Rreb1

UniProt

Q3UH06

RefSeq:AAX83010.1