Ribonuclease A

SKU: orb1944600

Description

Ribonuclease A (RNase A) is an endoribonuclease essential for RNA removal in molecular biology workflows. It is widely used in vitro for nucleic acid purification, RNA sequencing library prep, and in structural biology to study protein-RNA interactions.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 9001-99-4
• Target: Others
• Solubility: H2O:80 mg/mL

Bioactivity

• In Vitro:
  The most common application of Ribonuclease A is to remove RNA during plasmid DNA or genomic DNA preparation. Preparation of Ribonuclease A Stock Solution:Prepare a 10 mg/mL Ribonuclease A stock solution using 10 mM sodium acetate (pH 5.2).Heat at 100°C for 15 minutes. Cool to room temperature, add 1/10 (v/v) 1 M Tris-HCl (pH 7.4), and adjust the pH to 7.4.Store at -20°C for stable storage for up to 2 years. Note: Boiling the Ribonuclease A solution under neutral conditions may lead to the precipitation of RNase. Boiling at a lower pH, if precipitation occurs, may be due to the presence of protein impurities. In such cases, the precipitate can be removed by high-speed centrifugation (13,000 rpm).
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EC 3.1.27.5, RNase A, Ribonuclease A

Quick Database Links

Gene Symbol

Others