RHOC Rabbit Polyclonal Antibody

SKU: orb704253

Description

RHOC Rabbit Polyclonal Antibody

Research Area

Cell Biology, Ras Family, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human RHOC (1-193/193aa)
• Target: RHOC
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARH 9; ARH9; ARHC; H9; MGC1448; MGC61427; Oncogene RHO H9; Ras homolog gene family member C; RAS related homolog 9; RHO C; Rho cDNA clone 9; Rho related GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; rhoC; RhoC GTPase; RHOC_HUMAN; RHOH 9; RHOH9; Small GTP binding protein RhoC.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: RHOC knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-RHOC (orb704253) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: U2os (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: A431 (Human) Cell Lysate at 30 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-RHOC (orb704253) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

RHOC

UniProt

P08134