RFFL Antibody

• Catalog Number: orb344634
• Category: Antibodies
• Description: RFFL antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
• Antibody Type: Primary Antibody
• Concentration: 5.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:20,000, IHC: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: This protein A purified antibody is directed against human RFFL protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
• Conjugation: Unconjugated
• UniProt ID: Q8WZ73
• NCBI: 17432433
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-RFFL antibody, Rififylin, RING finger
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.
• Expiration Date: 12 months from date of receipt.

Biorbyt's Affinity Purified anti-RFFL antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Western blot using Biorbyt's Protein A Purified anti-RFFL antibody shows detection of RFFL (arrowhead) in lysate. Lanes correspond to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RFFL. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incuba-tion occurred for 1 h at room temperature.