RFFL antibody

• Catalog Number: orb344634
• Category: Antibodies
• Description: RFFL antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
• Concentration: 5.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:20,000, IHC: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: This protein A purified antibody is directed against human RFFL protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
• Conjugation: Unconjugated
• UniProt ID: Q8WZ73
• NCBI: 17432433
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-RFFL antibody, Rififylin, RING finger
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human liver tissue using RFFL antibody

Western blot analysis of 293T cell lysate using RFFL antibody