RET Antibody (N-term C166)

SKU: orb1929002

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This RET antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-182 amino acids from the N-terminal region of human RET. Antigen Region: 152-182 aa.
• Target: RET {ECO:0000303|PubMed:2660074, ECO:0000312|HGNC:HGNC:9967}
• Molecular Weight: 124319 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51

Western blot analysis of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

RET {ECO:0000303|PubMed:2660074, ECO:0000312|HGNC:HGNC:9967}

UniProt

P07949

RefSeq (Protein):NP_066124.1, NP_065681.1