Remodelin hydrobromide

SKU: orb1226038

Description

A potent inhibitor of the acetyl-transferase protein NAT10 that acetylates both histones and microtubules; significantly reduces the prevalence of misshapen nuclei in HGPS cells, prevents FTI-induced nuclear shape defects in normal fibroblasts and U2OS cells; does not affect Golgi apparatus integrity or tubulin polymer assembly, and does not cause cells to accumulate in mitosis.

Key Properties

• CAS Number: 1622921-15-6
• MW: 363.2754
• Purity: >98% (HPLC)
• Formula: C₁₅H₁₅BrN₄S
• SMILES: N#CC1=CC=C(C2=CSC(N/N=C3CCCC/3)=N2)C=C1.[H]Br
• Target: HDAC
• Solubility: DMSO: ≥ 44 mg/mL

Bioactivity

• In Vitro:
  Cell Proliferation Assay Cell line: Prostate cancer cell lines VCaP, PC3, and DU145. Concentration: 0, 10, 20, 40 μM. Incubation time: 1, 2, 7 days. Result: Inhibited NAT10 and suppressed the growth of both AR-positive and AR-negative prostate cancer cells. Displayed significantly decreased cell proliferation activity over time, compared to the control group. Decreased colony formation ability with a dose-dependent manner. Immunofluorescence Cell line: Prostate cancer cell lines VCaP, PC3, and DU145. Concentration: 20 μM. Incubation time: 24 h. Result: Showed a significant decrease in both the positive labeling rate and the fluorescence intensity compared to the control group. Significantly reduced both the staining foci of IdU and the staining foci of CldU compared to control group. Western blot analysis. Cell line: Skin fibroblasts from LmnaG609G/G609G and wildtype (WT) littermates. Concentration: 1 μM. Incubation time: 7 days. Result: Decreased the higher level of the DNA double-strand break (DSB) marker gamma H2AX (γH2AX, Ser-139 phosphorylated histone H2AX).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Remodelin HBr | Remodelin Hydrobromide

Quick Database Links

Gene Symbol

HDAC