Rat IgG1 isotype control Biotin Antibody

SKU: orb343820

Description

Rat IgG1 isotype control Biotin Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT
• Dilution Range: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Application Notes:
  RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Key Properties

• Isotype: IgG1
• Clone No.: RG1
• Purity: RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG1 confirmed by Dot Blot.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rat Isotype Control, Rat IgG1 Biotin isotype, RAT IgG1 subclass isotype control