Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit

SKU: orb3145704

Description

This Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1A.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1A in samples. An antibody specific for HIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

• Reactivity: Rat
• Sample Types: Cell culture supernatants#Serum#Plasma#Other biological fluids
• Range: Please inquire
• Sensitivity: Please inquire

Storage & Handling

• Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

HIF1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; hypoxia-inducible factor 1; alpha