RAD51 Antibody

• Catalog Number: orb1257126
• Category: Antibodies
• Description: RAD51 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAD51 (NP_002866.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37kDa
• Target: RAD51
• UniProt ID: Q06609
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RAD51A, RECA, DNA repair protein RAD51 homolog 1,
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RAD51 antibody (orb1257126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.