Rabbit polyclonal antibodies to Bradykinin

SKU: orb2284547

Description

Bradykinin (BK) is a peptide that promotes inflammation. It causes arterioles to dilate (enlarge) via the release of prostacyclin, nitric oxide, and endothelium-derived hyperpolarizing factor and makes veins constrict, via prostaglandin F2, thereby leading to leakage into capillary beds, due to the increased pressure in the capillaries. Bradykinin consists of nine amino acids, and is a physiologically and pharmacologically active peptide of the kinin group of proteins.

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: Western blot: 0.1-1.0 µg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG/IgM
• Immunogen: Bradykinin (RPPGFSPFR) acetate conjugated to Ovalbumin
• Target: Bradykinin
• Molecular Weight: 1060.21 Da
• Purification: Immunogen affinity column
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Glycine pH 7.2
• Concentration: Batch dependent within range 2.0-2.5 mg/mL
• Disclaimer: For research use only

Western blot demonstrating our polyclonal detecting Bradykinin-BSA conjugate at 1 in 2000 dilution. Lane 1: Marker; Lane 2: Bradykinin-BSA (5ng); Lane 3: Bradykinin-BSA (10ng); Lane 4: Bradykinin-BSA (50ng); Lane 5: Bradykinin-BSA (100ng); Lane 6: Blank; Lane 7: BSA (5ng); Lane 8: BSA (10ng); Lane 9: BSA (50ng); Lane 10: BSA (100ng).

ELISA Plates were coated with 4 µg/mL Bradykinin-BSA, rabbit anti-bradykinin was added at different concentrations and detected with an anti-rabbit HRP.

Quick Database Links

Gene Symbol

Bradykinin