Rabbit polyclonal antibodies to Bradykinin

• Catalog Number: orb2284547
• Category: Antibodies
• Description: Bradykinin (BK) is a peptide that promotes inflammation. It causes arterioles to dilate (enlarge) via the release of prostacyclin, nitric oxide, and endothelium-derived hyperpolarizing factor and makes veins constrict, via prostaglandin F2, thereby leading to leakage into capillary beds, due to the increased pressure in the capillaries. Bradykinin consists of nine amino acids, and is a physiologically and pharmacologically active peptide of the kinin group of proteins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG/IgM
• Immunogen: Bradykinin (RPPGFSPFR) acetate conjugated to Ovalbumin
• Antibody Type: Primary Antibody
• Concentration: Batch dependent within range 2.0-2.5 mg/mL
• Dilution range: Western blot: 0.1-1.0 µg/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 1060.21 Da
• Target: Bradykinin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Glycine pH 7.2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot demonstrating our polyclonal detecting Bradykinin-BSA conjugate at 1 in 2000 dilution. Lane 1: Marker; Lane 2: Bradykinin-BSA (5ng); Lane 3: Bradykinin-BSA (10ng); Lane 4: Bradykinin-BSA (50ng); Lane 5: Bradykinin-BSA (100ng); Lane 6: Blank; Lane 7: BSA (5ng); Lane 8: BSA (10ng); Lane 9: BSA (50ng); Lane 10: BSA (100ng).

ELISA Plates were coated with 4 µg/mL Bradykinin-BSA, rabbit anti-bradykinin was added at different concentrations and detected with an anti-rabbit HRP.