Rabbit IgG (H&L) Antibody

• Catalog Number: orb347772
• Category: Antibodies
• Description: Rabbit IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Gallus
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rabbit
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Rabbit IgG and Rabbit Serum.
• Immunogen: Rabbit IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Rabbit IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Chicken Anti Rabbit IgG Antibody, Chicken Anti-Rab
• Note: For research use only

Protective effects of Eda.B against NETs and inflammatory factors. (d–g) Protein levels of TNFα and citH3 at day 1 and day 3 after stroke. Representative bands are on the top.

Western Blot of Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n orb347772). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n orb347772) 1:1000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb346892) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.