Rabbit IgG (H&L) Antibody

SKU: orb347744

Description

Rabbit IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Reactivity: Rabbit
• Application Notes:
  Anti-RABBIT IgG (H&L) (SHEEP) Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rabbit IgG whole molecule
• Purity: Anti-RABBIT IgG (H&L) (SHEEP) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 2.1 mg/mL
• Disclaimer: For research use only

Alternative Names

Sheep Anti-Rabbit IgG, Rabbit Secondary Ab, anti-Rabbit IgG Antibody in Sheep

Western Blot of Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347744). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347744) 1:3000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n orb347953) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.