Rabbit TIF-IA Antibody

SKU: orb1525518

Description

TIF-IA Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 601 and C-terminus
• Target: TIF-IA
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A-270G1.2; RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog; TIFIA; TIF-IA; transcription initiation factor IA; transcription initiation factor TIF-IA

Detection of human TIF-IA by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TIF-IA antibody orb1525518 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TIF-IA was also immunoprecipitated by rabbit anti-TIF-IA antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

TIF-IA

UniProt

Q9NYV6

RefSeq (Protein):NP_060897.3