Rabbit TFIIS Antibody

SKU: orb1526980

Description

TFIIS Antibody

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-TFIIS antibody A302-239A.; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 200 and 250
• Target: TFIIS
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

epididymis secretory sperm binding protein; GTF2S; SII; TCEA; TF2S; TFIIS; transcription elongation factor A (SII), 1; transcription elongation factor A protein 1; transcription elongation factor S-II protein 1; transcription elongation factor TFIIS.o; transcription factor IIS

Detection of human and mouse TFIIS by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-TFIIS antibody orb1526980 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. TFIIS was successfully immunoprecipitated by rabbit anti-TFIIS antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Detection of human TFIIS by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TFIIS (Cat. No. orb1526980) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

TFIIS

UniProt

P23193

RefSeq (Protein):NP_958845.1