Rabbit SPF45 Antibody

SKU: orb1526518

Description

SPF45 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-SPF45 antibody A302-498A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 25 and 75
• Target: SPF45
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

45 kDa-splicing factor; RNA-binding motif protein 17; SPF45; splicing factor 45; splicing factor 45kDa

Detection of human SPF45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPF45 antibody orb1526518 used for IP at 6 µg per reaction.

Detection of human SPF45 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. orb1526518) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse SPF45 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. orb1526518) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SPF45

UniProt

Q96I25

RefSeq (Protein):NP_116294.1