Rabbit RNA Polymerase II Antibody

SKU: orb1523054

Description

RNA Polymerase II Antibody

Research Area

Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: ChIP, IP
• Dilution Range: WB - Not recommended. orb1523054 has not performed satisfactorily when used for WB of RNA Polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA Polymerase II.; IP - 2 - 10 µg/mg lysate; ChIP-PCR - 8 µg per IP
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 775 and 825
• Target: RNA Polymerase II
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody (orb1523054) used for IP at 6 µg per reaction.

Binding at pause site of the Jun B gene. Chromatin isolated from one 15 cm plate of HeLa cells was immunoprecipitated with 8.0 µg of anti-RNA polymerase II antibodies and PCR amplified.kerning24

Quick Database Links

Gene Symbol

RNA Polymerase II

UniProt

P24928

RefSeq (Protein):NP_000928.1