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Catalog Number | orb1530247 |
---|---|
Category | Antibodies |
Description | Reticulocalbin-1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 25 and 75 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Reticulocalbin-1 |
UniProt ID | Q15293 |
NCBI | NP_002892.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | PIG20; reticulocalbin-1; reticulocalbin 1, EF-hand Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse Reticulocalbin-1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody orb1530247 at 0.4 µg/ml.
Detection of human RCN1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human Reticulocalbin-1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody.
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |