Rabbit RelA Antibody

SKU: orb1527723

Description

RelA Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 501 and C-terminus
• Target: RelA
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

Detection of human and mouse RelA/p65 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RelA/p65 antibody orb1527723 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. RelA/p65 was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human RelA/p65 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RelA/p65 (Cat. No. orb1527723) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Localization of RelA Binding Sites by ChIP-sequencing. Chromatin from human ependymoma tumor was immunoprecipitated with anti-RelA antibody orb1527723 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RelA binding within a 250 Kb region of chromosome 19 as detected using anti-RelA orb1527723.

Quick Database Links

Gene Symbol

RelA

UniProt

Q04206

RefSeq (Protein):NP_068810.3