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Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 675 and 725 |
| Target | RECQ5 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human RECQ5 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RECQ5 antibody orb1526472 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. RECQ5 was also immunoprecipitated by rabbit anti-RECQ5 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_004250.4 |
|---|
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Protocol Information
Rabbit RECQ5 Antibody (orb1526472)
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