Rabbit anti-RanGAP1 Antibody

• Catalog Number: orb1527369
• Category: Antibodies
• Description: RanGAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 100 and 150
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RanGAP1
• UniProt ID: P46060
• NCBI: NP_002874.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Fug1; segregation distortion; segregation distorte
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody orb1527369 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody orb1527369 used for IP at 6 µg per reaction.

Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. orb1527369 (left) and orb1527367 (right) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB