Rabbit PUF60 Antibody

SKU: orb1525939

Description

PUF60 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 400 and 450
• Target: PUF60
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60 kDa poly(U)-binding-splicing factor; FBP interacting repressor; FBP-interacting repressor; FIR; FUSE-binding protein-interacting repressor; poly(U) binding splicing factor 60KDa; poly(U)-binding-splicing factor PUF60; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; RoBPI; Siah binding protein 1; Siah-binding protein 1; SIAHBP1; Siah-BP1; VRJS

Detection of human PUF60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody orb1525939 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human and mouse PUF60 by immunohistochemistry.Sample: FFPE section of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PUF60 (Cat. No. orb1525939) used at a dilution of 1: 200 (1µg/ml) Detection: DAB

Quick Database Links

Gene Symbol

PUF60

UniProt

Q9UHX1

RefSeq (Protein):NP_510965.1