Rabbit PGP Antibody

SKU: orb1520653

Description

PGP Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µl/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 271 and C-terminus
• Target: PGP
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

aspartate-based ubiquitous Mg(2+)-dependent phosphatase; AUM; G3PP; glycerol-3-phosphate phosphatase; PGP; PGPase; Phosphoglycolate phosphatase

Detection of human PGP by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, HEK293T, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGP antibody (orb1520653) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse PGP by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGP antibody (orb1520653) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PGP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PGP antibody (orb1520653) used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

PGP

UniProt

A6NDG6

RefSeq (Protein):NP_001035830.1