Rabbit anti-PCNA Antibody

SKU: orb1530431

Description

PCNA Antibody

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 75 and 125
• Target: PCNA

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

proliferating cell nuclear antigen; PCNA; DNA polymerase delta auxiliary protein; ATLD2; cyclin

Detection of human and mouse PCNA by western blot.Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody orb1530431 at 0.1 µg/ml.

Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:10, 000 (0.1µg/ml).

Detection of human PCNA by WB and IP.Samples: A. A-431 cells. B. MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody used at the indicated concentrations for WB.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_872590.1