Rabbit anti-PCNA Antibody

• Catalog Number: orb1530431
• Category: Antibodies
• Description: PCNA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 75 and 125
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PCNA
• UniProt ID: P12004
• NCBI: NP_872590.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: proliferating cell nuclear antigen; PCNA; DNA poly
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse PCNA by western blot.Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody orb1530431 at 0.1 µg/ml.

Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:10, 000 (0.1µg/ml).

Detection of human PCNA by WB and IP.Samples: A. A-431 cells. B. MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody used at the indicated concentrations for WB.