Rabbit PBE Antibody

SKU: orb1524873

Description

PBE Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 673 and C-terminus
• Target: PBE
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; ECHD; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; FRTS3; L-3-hydroxyacyl-CoA dehydrogenase; LBFP; L-bifunctional protein, peroxisomal; LBP; L-PBE; PBE; PBFE; peroxisomal bifunctional enzyme; peroxisomal enoyl-CoA hydratase

Detection of human PBE by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PBE antibody orb1524873 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PBE was also immunoprecipitated by rabbit anti-PBE antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Quick Database Links

Gene Symbol

PBE

UniProt

Q08426

RefSeq (Protein):NP_001957.2