Rabbit anti-NPAT Antibody

• Catalog Number: orb1526020
• Category: Antibodies
• Description: NPAT Antibody
• Target: NPAT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 1377 and C-terminus
• UniProt ID: Q14207
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: nuclear protein of the ataxia telangiectasia mutat
• Note: For research use only
• NCBI: NP_002510.2

Detection of human NPAT by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NPAT antibody orb1526020 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human NPAT by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. orb1526020) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB