You have no items in your shopping cart.
Cart summary
Item 1 of 1
Rabbit anti-NELFE Antibody
Catalog Number: orb1527565
| Catalog Number | orb1527565 |
|---|---|
| Category | Antibodies |
| Description | NELFE Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 75 and 125 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 5 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | NELFE |
| UniProt ID | P18615 |
| NCBI | NP_002895.3 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | negative elongation factor polypeptide E; RNA-bind Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human NELFE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody orb1527565 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NELFE was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
- Item 1 of 2
- Item 1 of 2
- Item 1 of 1
