Rabbit anti-NELFE Antibody

SKU: orb1527563

Description

NELFE Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution range: WB - Not recommended. A301-914A has not performed satisfactorily when used for WB of NELFE in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NELFE.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 330 and C-terminus
• Target: NELFE
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

negative elongation factor polypeptide E; RNA-binding protein RD; RDP; RDBP; RD RNA-binding protein; RD; NELF-E; negative elongation factor E; major histocompatibility complex gene RD; D6S45; nuclear protein

Detection of human NELFE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody orb1527563 used for IP at 3 µg/mg lysate.

Detection of human NELFE by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NELFE (Cat. No. orb1527563) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NELFE

UniProt

P18615

RefSeq (Protein):NP_002895.3