Rabbit Lactate Dehydrogenase B/LDHB Antibody

SKU: orb1522363

Description

Lactate Dehydrogenase B/LDHB Antibody

Research Area

Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 284 and C-terminus
• Target: Lactate Dehydrogenase B/LDHB
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

epididymis secretory protein Li 281; HEL-S-281; lactate dehydrogenase H chain; LDH heart subunit; LDH-B; LDHBD; LDH-H; L-lactate dehydrogenase B chain; renal carcinoma antigen NY-REN-46; testicular secretory protein Li 25; TRG-5

Detection of human and mouse Lactate Dehydrogenase B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody orb1522363 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody orb1522363 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

Lactate Dehydrogenase B/LDHB

UniProt

P07195

RefSeq (Protein):NP_002291.1