Rabbit Gamma-Tubulin Antibody

SKU: orb1526284

Description

Gamma-Tubulin Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 401 and C-terminus
• Target: Gamma-Tubulin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDCBM4; Gamma-1-tubulin; gamma-tubulin complex component 1; GCP-1; TUBG; TUBGCP1; tubulin gamma-1 chain; tubulin, gamma polypeptide

Detection of human and mouse Gamma-tubulin by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Gamma-tubulin antibody orb1526284 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Gamma-tubulin was also immunoprecipitated by rabbit anti-Gamma-tubulin antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

Gamma-Tubulin

UniProt

P23258

RefSeq (Protein):NP_001061.2