Rabbit anti-eIF4G1/eIF4GI Antibody

• Catalog Number: orb1527811
• Category: Antibodies
• Description: eIF4G1/eIF4GI Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 550 and 600
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF4G1/eIF4GI
• UniProt ID: Q04637
• NCBI: NP_886553.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: eIF-4-gamma 1; PARK18; P220; eukaryotic translatio
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human eIF4G1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-eIF4G1 antibody orb1527811 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.

Detection of human eIF4G1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-eIF4G1 antibody orb1527811 used for IP at 3 µg/mg lysate.

Detection of human eIF4G1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4G1 (Cat. No. orb1527811) used at a dilution of 1: 200 (1µg/ml). Detection: DAB