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Rabbit DJ-1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 139 and C-terminus |
| Target | DJ-1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human DJ-1 by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-DJ-1 antibody orb1529703 at 0.1 µg/ml.

Detection of human and mouse DJ-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DJ-1 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human DJ-1 by WB and IP/Mass Spectrometry.Samples: A) HeLa, MCF-7, HEK293T cells. B) Cytosolic and nuclear extract from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-DJ-1 antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_009193.2 |
|---|
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Protocol Information
Rabbit DJ-1 Antibody (orb1529703)
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