Rabbit anti-DJ-1 Antibody

• Catalog Number: orb1529703
• Category: Antibodies
• Description: DJ-1 Antibody
• Target: DJ-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 139 and C-terminus
• UniProt ID: Q99497
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: protein/nucleic acid deglycase DJ-1; protein DJ-1;
• Note: For research use only
• NCBI: NP_009193.2

Detection of human DJ-1 by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-DJ-1 antibody orb1529703 at 0.1 µg/ml.

Detection of human and mouse DJ-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DJ-1 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human DJ-1 by WB and IP/Mass Spectrometry.Samples: A) HeLa, MCF-7, HEK293T cells. B) Cytosolic and nuclear extract from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-DJ-1 antibody.