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Rabbit Cytoskeletal Actin Antibody
SKU: orb1530126
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:2,000 - 1:10,000. Epitope retrieval may not be necessary. In some instances epitope retrieval with citrate buffer pH 6.0 may enhance staining for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Xenopus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | Cytoskeletal Actin |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACT; ACTG; actin, cytoplasmic 2; cytoskeletal gamma-actin; DFNA20; DFNA26; epididymis luminal protein 176; Gamma-actin; HEL-176
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Detection of human and mouse Actin by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody orb1530126 at 0.1 µg/ml.
Detection of human Actin by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1, 000 (1µg/ml).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001605.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Rabbit Cytoskeletal Actin Antibody (orb1530126)
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