Rabbit beta Actin Antibody

SKU: orb1530115

This product can be custom made

Description

Cytoskeletal Actin Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 3

Tested Applications	IHC, WB
Dilution Range	WB - 1:10,000 - 1:25,000; IP - Not recommended; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Frog, Gallus, Golden Hamster, Guinea pig, Porcine, Rabbit, Rat, Sheep
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	Cytoskeletal Actin
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1

Beta-Actin Rabbit Polyclonal Antibody (Loading Control) [orb500817]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Feline, Fish, Gallus, Guinea pig, Hamster, Insect, Porcine, Rabbit, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl, 500 μl, 200 μl, 1 ml

Item 1 of 10
ACTB Antibody [orb420309]
Applications:
ELISA, IF, KO/KD Validated, WB
Reactivity:
Frog, Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 200 μg

Quality Guarantee

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Detection of mouse beta Actin by western blot.Samples: BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody orb1530115 at 0.04 µg/ml.

Detection of human beta Actin by western blot.Samples: Whole cell lysate from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, MOLT-4, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody orb1530115 at 0.04 µg/ml.

Detection of human beta Actin by immunohistochemistry. Sample: FFPE section of human prostate cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin used at 1:1, 000 (1µg/ml).

Quick Database Links

Gene Symbol

Cytoskeletal Actin

UniProt

P60709

RefSeq (Protein):NP_001092.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001092.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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