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Rabbit beta Actin Antibody
SKU: orb1530115
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:10,000 - 1:25,000; IP - Not recommended; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Frog, Gallus, Golden Hamster, Guinea pig, Porcine, Rabbit, Rat, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | Cytoskeletal Actin |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1
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Detection of mouse beta Actin by western blot.Samples: BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody orb1530115 at 0.04 µg/ml.
Detection of human beta Actin by western blot.Samples: Whole cell lysate from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, MOLT-4, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody orb1530115 at 0.04 µg/ml.
Detection of human beta Actin by immunohistochemistry. Sample: FFPE section of human prostate cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin used at 1:1, 000 (1µg/ml).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001092.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Rabbit beta Actin Antibody (orb1530115)
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