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Rabbit CBX2 Antibody
Description
Images & Validation
−| Tested Applications | ChIP, IP |
|---|---|
| Dilution Range | WB - Not recommended. orb1526470 has not performed satisfactorily when used for WB of CBX2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CBX2.; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 482 and C-terminus |
| Target | CBX2 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human CBX2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX2 antibody orb1526470 used for IP at 6 µg per reaction.

Localization of CBX2 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX2 antibody orb1526470 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX2 binding within a 1.5 Mb region of chromosome 1 as detected using anti-CBX2 antibody orb1526470.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005180.1 |
|---|
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Protocol Information
Rabbit CBX2 Antibody (orb1526470)
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