QX77

SKU: orb1222596

Description

QX77 is a chaperone-mediated autophagy (CMA) activator.

Key Properties

• CAS Number: 1798331-92-6
• MW: 300.74
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₃ClN₂O₂
• SMILES: CC(=O)Nc1ccc(cc1)C2=Nc3ccc(Cl)cc3OC2
• Target: HIF/HIF Prolyl-hydroxylase
• Solubility: DMSO : ≥ 64 mg/mL; 212.81 mM

Bioactivity

• In Vitro:
  QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/- MEFs. In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells. Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane. QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells. QX77 protects cystinotic cells from the increased susceptibility totert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression. QX77 (10 μM; 0, 3 or 6 days) activates CMA and increases LAMP2A expression inD3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology. Western blot analysis. Cell line: ES D3 cell line; E14TG2a (E14) cell line. Concentration: 10 μM. Incubation time: 3 or 6 days. Result: Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

HIF/HIF Prolyl-hydroxylase