Puerarin

SKU: orb1304986

Description

Puerarin

Research Area

Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 3681-99-0
• MW: 416.38
• Purity: 98.90% (May vary between batches)
• Formula: C₂₁H₂₀O₉
• SMILES: OC=1C(=C2C(C(=O)C(=CO2)C3=CC=C(O)C=C3)=CC1)[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O
• Target: 5-HT Receptor
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:250 mg/mL (600.41 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (12.01 mM)

Bioactivity

• In Vivo:
  Puerarin, at a dose of 25 μM, induces dose-dependent growth inhibition in HT-29 cells. This effect is accompanied by an increase in bax and a decrease in c-myc and bcl-2.
• In Vitro:
  Administering 300 mg/kg of Puerarin daily through oral ingestion to rats on a high-cholesterol diet significantly reduces the increase in total cholesterol levels in both serum and liver caused by the high-cholesterol diet.
• Cell Research:
  RAW264.7 cells are maintained at subconfluence in 95% air and 5% CO2 humidified atmosphere maintained at 37°C. The medium used for routine subculture is Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin (100 units/mL) and streptomycin (100 μg/ mL). An MTT assay is used to measure the viability of the cells after treatment with puerarin. After the supernatants are removed for nitrite determination, cells are incubated at 37°C with MTT (0.05 mg/mL) for 4 h, and the optical density is measured at 540 nm. The concentrations of puerarin are10, 20, 40 and 100 μM.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serotonin Receptor, Puerarin, 5-HT Receptor, 5-hydroxytryptamine Receptor, 5HTReceptor, 5HT Receptor, 5-HT, Inhibitor, inhibit, Kakonein

Compound Identifiers

PubChem CID

5281807