PTEN Antibody (N-term)

• Catalog Number: orb1928503
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PTEN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-104 amino acids from the N-terminal region of human PTEN.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P60484
• MW: 47166 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB05082
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phosphatidylinositol 3,5-trisphosphate 3-phosphata
• Note: For research use only
• NCBI: NP_000305.3
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTEN antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PTEN Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 ug/lane).This demonstrates the PTEN antibody detected the PTEN protein (arrow).

Western blot analysis of PTEN (arrow) using rabbit PTEN Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTEN gene (Lane 2).

Confocal immunofluorescent analysis of PTEN Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).