PSD95 Antibody (RPE)

SKU: orb147012

Description

Mouse monoclonal to PSD95 (RPE). Postsynaptic Density protein 95 (PSD95), also known as Postsynaptic Density protein 95 (PSD95), also known as...

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, WB
• Dilution range: WB (1:250), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 6G6
• Immunogen: Recombinant rat PSD-95
• Target: PSD95
• Molecular Weight: 75kDa
• Purification: Protein A Purified
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

PSD 95, PSD-95, PSD95, DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.

Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

PSD95

UniProt

P31016

NCBI Gene ID

29495

RefSeq (Protein):NP_062567.1