PSAP antibody

SKU: orb2276501

Description

PSAP antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Prostate: A strong PSAP immunostaining should, be seen in all glandular cells. Negative Control: Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MSVA-452M
• Immunogen: Recombinant full-length human ACPP protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

5′-nucleotidase (5′-NT); Acid phosphatase prostate; ACP3; Ecto-5′-nucleotidase; Prostatic acid phosphatase (PAP); Prostatic acid phosphatase; Thiamine monophosphatase (TMPase)

Appendix, mucosa: Complete absence of PSAP immunostaining in all epithelial and non-epithelial cell types of the appendix.

Prostate Prostatic adenocarcinoma Gleason 448 with strong diffuse PSAP positivity.

Strong PSAP immunostaining in all acinar epithelial cells of the prostate.

Quick Database Links

UniProt

P15309