Prostein antibody

SKU: orb2276238

Description

Prostein antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Stomach mucosa: At least a moderate, granular, cytoplasmic prostein positivity should be seen in surface epithelial cells. Negative Control: Stomach mucosa: Prostein immunostaining should be absent in gastric glands. Cellular Localization: Membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-460R
• Immunogen: Synthesized peptides to the N terminus of human SLC45A3 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

SLC45A3, PCANAP6, Prostate Cancer Associated Protein 6, IPCA-6

Normal prostate with strong granular perinuclear cytoplasmic prostein staining of luminal cells.

Prostatic adenocarcinoma Gleason 336 with strong granular perinuclear cytoplasmic prostein staining of tumor cells.

Stomach mucosa with granular cytoplasmic prostein staining of surface epithelial cells.

Quick Database Links

UniProt

Q96JT2