Proliferation Marker IPO-38 Antibody

SKU: orb1535171

Description

Proliferation Marker IPO-38 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-Fr, IHC-P, WB
• Dilution Range: ELISA (1:10000 - 1:50000), IHC, IHC-Fr, IHC-P (10 µg/ml), WB (1:500 - 1:2000)
• Reactivity: Human
• Application Notes:
  Further information: This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized). An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: IPO-38
• Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
• Target: Proliferation Marker IPO-38
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Proliferation Marker IPO-38