Progesterone Receptor antibody

SKU: orb2276399

Description

Progesterone Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Positive Control: Uterine cervix: almost all columnar epithelial cells, basal squamous epithelial cells and most of the stromal cells must show a strong nuclear staining with only minimal cytoplasmic staining. Negative Control: Tonsil: PR immunostaining should be absent in all epithelial and non-epithelial cells. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-570R
• Immunogen: Recombinant fragment (around aa 1-200) of human PGR (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

R3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B

Endometroid carcinoma of the ovary depicting a strong PR immunostaining of all tumor cells.

Invasive breast cancer of no special type NST with strong PR immunostaining in 100% of tumor cells.

Uterus endocervix Strong PR immunostaining in epithelial and stromal cells of the endocervix.

Quick Database Links

UniProt

P06401