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Tested Applications
WB 42971ELISA 22359SDS-PAGE 15109Blocking 11025IHC 2121MS 2019HPLC 620CLIA 507LF 491EIA 382Cell Culture 288FA 199IP 98IF 88ChIP 57DOT 49IA 38Functional Studies 24FC 21In vitro 16In vivo 15ATPase Activity Assay 9CBA 6Control 6ICC 6Purification 6FACS 2GICA 2IAC 2IB 2ICA 2PCR 2SBR 22D-PAGE 1CoIP 1DARTs Assay 1Double Diffusion 1ELISpot 1FLISA 1HAI 1ID 1IEP 1RIA 1SB 1Turbidimetry 1cDNA Synthesis 1
Reactivity
Human 16904Mouse 6095Rat 4647Monkey 1685Bovine 1676Porcine 1085Canine 675Gallus 657Rabbit 425Zebrafish 387Virus 299Sheep 297Other 56Bacteria 36Hamster 10Goat 9Feline 8Plant 8Guinea pig 7Equine 5Camelus 3E. coli 3H. pylori 2Parasite 2Primate 2Protozoa 2Rat and Mouse 2A. thaliana 1Algae 1Aspergillus 1Ferret 1Fungi 1HCMV 1HSV-1 1Jellyfish 1Marmoset 1Rabies virus 1Yeast 1
Predicted Reactivity
Conjugation
Biologically Active
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15.3 KDa. Observed: 16-22 KDa, reducing conditions
Human
Ile56-Glu182
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 22.5 KDa. Observed: 30-60 KDa, reducing conditions
Human
Phe29-Arg215
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36 KDa. Observed: 50-75 KDa, reducing conditions
Human
Asp35-Ser353
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 50.54 KDa. Observed: 88 KDa, reducing conditions
Human
Gln28-Glu480
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified)
Predicted: 14.76 KDa. Observed: 30-45 KDa, reducing conditions
Human
Gln19-Pro139
C-6His
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.83 KDa. Observed: 50-65 KDa, reducing conditions
Human
Cys35-Asp328
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.2 KDa. Observed: 28 KDa, reducing conditions
Human
Glu21-Arg193
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 21.61 KDa. Observed: 25-32 KDa, reducing conditions
Human
Ala36-Ile218
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 74.1 KDa. Observed: 90-110 KDa, reducing conditions
Human
Glu17-Thr434
C-6His-Flag-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 23.4 KDa. Observed: 32-50 KDa, reducing conditions
Human
Ser23-Met226
C-6His
10 μg, 500 μg, 1 mg, 50 μg