PRMT5 Antibody

SKU: orb1248607

Description

PRMT5 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Jurkat lysates (calculated MW of 72.7kDa and 71.3kDa according to NP_006100.2; NP_001034708.1). Recommended concentration: 1-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: RACREKDRDPEAQ
• Target: PRMT5
• Molecular Weight: Approx 70kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRMT5, protein arginine methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog

orb1248607 staining (2 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

PRMT5

UniProt

O14744

RefSeq (Protein):NP_006100.2, NP_001034708.1